We have established models of deep wound infection in mice, guinea pigs, rats and rabbits. Among them, taking mice as an example, we first used a biopsy punch to form a 6mm deep wound on the back surface of the thoracic spine of the mouse, and then directly inoculated the test bacteria on the wound surface to form an infected deep wound model. After that, the tested candidate compound can be injected into the mouse body by topical, intragastric, subcutaneous, intraperitoneal or intravenous injection, and then observe and analyze it to evaluate the antibacterial effect of the candidate. Each research parameter (animal strains, pathogens, comparators and dosing schedules, etc.) in the process can be customized to perfectly meet all the needs of the customer's research project.