Since many of the tools currently used to study microglia cannot distinguish between microglia and central nervous system macrophages, this limitation has triggered a new wave of innovation in tools for studying microglia in vivo and in vitro. Creative Biolabs, for the first time, is able to specifically manipulate a single glial cell type and provides a novel cell culture tool to achieve the optimal primary microglia cell isolation while maintaining the survival of glial cells without any fundamental function change. The combination of this novel tool with single-cell sequencing will help researchers continue to uncover the mysteries and magic of these important cells.